Journal: Journal of Advanced Research

Article Title: Increased Rab1a accelerates osteoarthritis by inhibiting autophagy via activation of the mTORC1-S6K pathway

doi: 10.1016/j.jare.2024.11.009

Figure Lengend Snippet: Rab1a exaggerated extracellular matrix degradation and apoptosis in mice. A) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Rab1a AAV or control AAV were exhibited via immunohistochemical analysis, Scale bar, 20 μm. B) Protein deposition of Rab1a, MMP13, ADAMTS5, and Aggrecan in articular cartilage with transfection by Ad-Rab1a or Ad-NC were detected using immunohistochemical analysis, scale bar, 20 μm. C) Quantification of A. n = 6. D) Quantification of B. n = 6. E, F) TUNEL staining of chondrocytes in hyaline cartilage (HC) and calcified cartilage (CC) of each group. Blue fluorescence (DAPI) represented the nucleus, and green fluorescence (fluorescein-dUTP) represented TUNEL-positive cells. Scale bar, 25 μm. G, H) Quantification of TUNEL-positive chondrocytes relative to total chondrocytes was shown. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. TUNEL: TdT-mediated dUTP Nick-End Labeling.

Article Snippet: Subsequently, The TUNEL reaction mixture was applied at room temperature for 1 h. This was followed by coloring the nuclei with DAPI Staining Solution(Beyotime, China).

Techniques: Transfection, Control, Immunohistochemical staining, TUNEL Assay, Staining, Fluorescence, End Labeling